Takara_双酶切buffer用表

Note:

1) It is confirmed that 10 units of each enzyme completely digest 1 μg of DNA at 37°C in one

hour in 50 μl reaction mixture.

2) The concentration of Glycerol should be less than 10% to minimize star activity.

3) DNA may not be digested completely, when recognition sequences of two enzymes are

close each other, or when DNA takes high-structure conformation.

Relative activity for each reaction buffer

: supplied, activity measured buffer

: recommended buffer

Compositions of universal buffer

Instructions for use

Universal Buffers are 10× concentration. Please add 1/10 volume of the reaction mixture.

Since 10× T buffer does not contain BSA, be sure to add BSA to a final concentration of 0.01%.

Some restriction enzymes are supposed to exhibit a 100% activity when BSA or Triton X-100 is added to the reaction system. Since BSA or Triton X-100 supplied with enzymes is 10× concentration (0.1%), be sure to add 1/10 volume of the reaction mixture like buffer before starting the reaction.

Instructions for use

Add > 1/10 volume of 10× Loading Buffer to stop enzyme reaction and apply on agarose gel electrophoresis. SDS may precipitate during the storage at room temperature. In case precipitates generate, dissolve in warm bath before use.