酵母源金属硫蛋白对慢性汞中毒小鼠氧化损伤的修复作用

242 2016, V ol.37, No.15食品科学※营养卫生酵母源金属硫蛋白对慢性汞中毒小鼠

氧化损伤的修复作用

王颖1,2，王欣卉1，徐炳政3，张东杰1

（1.黑龙江八一农垦大学食品学院，黑龙江大庆 163319；2.国家杂粮工程技术研究中心，黑龙江大庆 163319；

3.青岛琅琊台集团股份有限公司，山东青岛 266400）

摘?要：探讨具有自主知识产权的两种酵母源金属硫蛋白（metallothionein，MT）亚型（MT-1和MT-2）对慢性汞中毒小鼠氧化损伤的修复作用。实验小鼠经氯化汞染毒，连续灌胃两种酵母源MT及二巯基丙磺酸钠（dimercaptopropansulfonate sodium，DMPS）28 d后测定小鼠体质量、全血汞含量及血清超氧化物歧化酶（superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力，丙二醛（malondialdehyde，MDA）含量及机体总抗氧化能力（total antioxidative capacity，T-AOC）。与正常对照组小鼠相比，模型对照组小鼠体质量显著下降（P＜0.05），灌胃给药28 d后，两种酵母源MT亚型高剂量组小鼠体质量显著升高（P＜0.05）；各给药组小鼠血液、肝脏及肾脏汞含量显著低于模型对照组（P＜0.05），两种酵母源MT亚型高剂量组的排汞效果显著优于DMPS组（P＜0.05）；各给药组小鼠血清中GSH-Px、SOD活力和MDA水平及总抗氧化能力接近正常对照组小鼠水平（P＞0.05），其中酵母源MT高剂量组的作用效果最佳，且在改善机体T-AOC方面的效果优于DMPS组（P＜0.05），酵母源MT-1亚型升高小鼠血清SOD活力和降低血清MDA含量的作用效果明显好于酵母源MT-2亚型。两种酵母源MT对慢性汞中毒小鼠具有良好的氧化损伤保护作用及排汞效果。

关键词：酵母源金属硫蛋白；汞；氧化损伤；保护

Metallothioneins from Yeast Expell Mercury and Protect against Oxidative Damage in Mice

Chronically Treated with Mercury

WANG Ying1,2, WANG Xinhui1, XU Bingzheng3, ZHANG Dongjie1

(1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. National Coarse Cereals

Engineering Research Center, Daqing 163319, China; 3. Qingdao Langyatai Co. Ltd., Qingdao 266400, China)

Abstract: The intent of this research was to explore the effect of metallothioneins from yeast with self-owned intellectual property rights on protecting against oxidative damage and expelling mercury in mice with chronic mercury poisoning. The mice with mercuric chloride exposure were continuously lavaged with two types (MT-1 and MT-2) of metallothioneins from yeast and dimercaptopropansulfonate sodium (DMPS) for 28 days, respectively. Body weight, mercury level in the whole blood, SOD, GSH-Px and MDA levels in serum, and total antioxidative capacity (T-AOC) in the body were measured at the end of the administration period. Compared with the normal control group, body weight in the model control group significantly decreased (P < 0.05), while both high-dose MT treatment groups showed a significant increase in body weight after gavaging for 28 days (P < 0.05). The mercury levels in blood, liver and kidney in all drug treatment groups were decreased signi?cantly when compared with the model control group (P < 0.05). The effects of two types of high-dose metallothioneins on expelling mercury were signi?cantly better than that of DMPS (P < 0.05). SOD, GSH-Px, MDA levels in serum and T-AOC in the body in all administration groups were brought back to the levels of the normal group (P > 0.05). Two monomeric forms of MT at the high dose had signi?cant impact on improving total antioxidant capacity in the body better than that of DMPS (P < 0.05).

However, MT-1 was signi?cantly superior to MT-2 in increasing serum SOD and reducing serum MDA. Both metallothioneins from yeast had an obvious effect on expelling mercury and protecting against oxidative damage.

Key words: metallothioneins from yeast; mercury; oxidative damage; protection

DOI:10.7506/spkx1002-6630-201615041

收稿日期：2016-04-07

基金项目：黑龙江省博士后资助项目（LBH-Z13168）；黑龙江省自然科学基金项目（C201445）；黑龙江省农垦总局“十二五”重点科技计划项目（HNK125B-13-05）；黑龙江省新世纪优秀人才培养计划项目（2014-2016）

作者简介：王颖（1979—），女，副教授，博士，研究方向为农产品加工与贮藏工程及食品质量安全。E-mail：Wychen156@https://www.sodocs.net/doc/391136756.html,