RAF265_LCMS_22321_MedChemExpress

File ..32\1\DATA\20161102\BIZ\BIZ-2016-N02-WJ4-1.D Tgt Mass (EZX): 518.00 Injection Date : 3 Nov 16 9:10 am +0800 Seq. Line : 0 Sample Name : BIZ-2016-N02-WJ4-1 Location : P2-F-05 Acq. Operator : KFF(LCMS-02) Inj : 1 Spec. Reported : MS Integration Inj Volume : 1 ul Acq. Method : D:\Chem32\1\METHODS\1-POS-3MIN.M

Analysis Method : D:\CHEM32\1\METHODS\1-POS-3MIN-PROSRSS.M

Catalog No : HY-10248 Batch#22321 Easy-Access Method: '1-POS-3MIN' 518.00 Method Info : Mobile Phase: A: water(0.01%TFA) B:ACN(0.01%TFA) Gradient: 5% to 95%B within 1.3 min Flow Rate :1.8ml/min

Column :SunFire C18, 4.6*50mm,3.5um A-RP-186

Oven Temperature : 45C

min*

0.5

1

1.5

2 2.5mAU 0

500100015002000*DAD1 B, Sig=214,4 Ref=off

1.6831.754

1.837

min*

0.51

1.5

2 2.5mAU 0

5001000*DAD1 C, Sig=254,4 Ref=off 1.6831.754

min

0.51 1.5

2

2.5

100000

200000300000400000 MSD1 TIC, MS File ES-API, Pos, Scan, Frag: 70

1.676

min

0.5

1

1.5

2

2.5

50000

100000 Ion 519, MSD1 519,Target Mass 518 +H Positive, EIC=518.7:519.7

1.678

Integration Results for DAD1 B, Sig=214,4 Ref=off

RetTim Width Area Height Area% MS(+)|-------|--------|--------------|--------------|--------|------| 1.68 0.02 2506.45 2456.51 99.40 260 1.75 0.02 11.94 10.32 0.47 260 1.84 0.02 3.08 3.05 0.12 233

Integration Results for DAD1 C, Sig=254,4 Ref=off

RetTim Width Area Height Area% MS(+)|-------|--------|--------------|--------------|--------|------| 1.68 0.01 1542.37 1567.09 99.34 260 1.75 0.02 10.18 9.31 0.66 260

Ret. Time: 1.68 <<<< POSITIVE SPECTRA >>>>

m/z

200

300

400

500

600

700

800

900

10000

2000030000

4000050000600007000080000 ES-API Positive

233.3

520.2

519.2

260.2